Analysis of Microplastics in Assorted Tea Ingredients Available in Wah Cantt Pakistan
Keywords:
Tea, whitener, milk, microplasticsAbstract
Microplastics are considered as one of the most widespread environmental pollutants. The undertaken study presents an analysis of microplastics polymer in tea, powder milk and tea whitener in Wah Cantt Pakistan. Twenty-three samples of local and branded tea, powder milk and tea whitener were analyzed. Samples were examined via FTIR and SEM-EDX to detect presence of microplastics polymer. FTIR spectra of all samples revealed presence of various microplastic such as polyethylene, polyethylene terephthalate, polyvinyl alcohol, polystyrene, nylon, along with other toxic contaminants such as chromium hydroxide, phosphorus, polyamide. Moreover, SEM images show irregular, rod shape, fragments, thread like structure which were commonly observed in microplastics. The EDX spectra have further authenticated SEM results as it has shown carbon dominant peaks which are common attributes of microplastics polymers. Comparatively, local samples are more contaminated compared to branded samples. The microplastics polymers found in tea ingredients are reportedly highly toxic and capable of damaging nervous system, immune system, hormonal imbalances and causing cancer. The study recommends that use of plastic should be minimized particularly for packaging of tea, powder milk and tea whitener.
References
Adnan, M., Ahmad, A., Ahmed, A., Khalid, N., Hayat, I., Ahmed, I., 2013. Chemical composition and sensory evaluation of tea (Camellia sinensis) commercialized in Pakistan. Pakistan Journal of Botany, 45(3), 901-907.
Afrin, S., Rahman, M. M., Hossain, M. N., Uddin, M. K., Malafaia, G., 2022. Are there plastic particles in my sugar? A pioneering study on the characterization of microplastics in commercial sugars and risk assessment. Science of the Total Environment, 837, 155849.
Alam, M. A., Mondal, A. K., Uddin, M. T., Razzaq, M. A., Chowdhury, M. J., Saha, M. S., 2023. Chemical Investigation and Separation of Chromium from Chrome Cake of BSCIC Tannery Industrial Estate at Hemayetpur, Dhaka, Bangladesh. Journal of Environmental and Public Health, 2023.
Amir, M., Ahmad, N., Sarfaroz, M., Ahmad, W., Ahmad, S., Mujeeb, M., Pottoo, F. H., 2019. Tamarindus indica fruit: Pharmacognostical standardization, detection of contaminant, and in vitro antioxidant activity. Journal of Pharmacy and Bio Allied Sciences, 11(4), 355.
Andrady, A. L., 2011. Microplastics in the marine environment. Marine Pollution Bulletin, 62(8), 1596-1605.
Bellamri, M., Brandt, K., Cammerrer, K., Syeda, T., Turesky, R. J., Cannon, J. R., 2023. Nuclear DNA and mitochondrial damage of the cooked meat carcinogen 2- amino-1-methyl-6-phenylimidazo [4, 5- b] pyridine in human neuroblastoma cells. Chemical Research in Toxicology, 36(8), 1361-1373.
Bo, L. J., Li, B., Zhang, K., Ma, R. H., Li, Y., Wang, Y. Q., Liu, Y. Y., 2023. Distribution, sources, and behavioral characteristics of microplastics in farmland soil. Huan Jing Ke Xue, 44(4), 2375-2383.
Butt, I., Fatima, M., Bhalli, M. N. U. M., Ali, M., 2020. Evaluation of drinking water quality and water borne disease prevalence in children at Shah di Khoi, Lahore, Pakistan. Journal of Himalayan Earth Science, 53(1), 118-125.
Chang, A. R., Anderson, C., 2017. Dietary phosphorus intake and the kidney. Annual Review of Nutrition, 37, 321-346.
Dharmaratne, R. W., 2019. Exploring the role of excess fluoride in chronic kidney disease: a review. Human and Experimental Toxicology, 38(3), 269-279. doi:10.1177/0960327118814161.
Furlani, R. P., Dias, F. F., Nogueira, P. M., Gomes, F. M., Tfouni, S. A., Camargo, M.C. 2015. Occurrence of macrocyclic lactones in milk and yogurt from Brazilian market. Food Control, 48, 43-47.
Geyer, R., 2020. Chapter 2-Production, use, and fate of synthetic polymers. In: Trevor, M.L. (eds) Plastic waste and recycling. Academic Press, 13-32. https://doi.org/10.1016/B978-0-12- 817880-5.00002-5.
Hernandez, L. M., Xu, E. G., Larsson, H. C., Tahara, R., Maisuria, V. B., Tufenkji, N., 2019. Plastic teabags release billions of microparticles and nanoparticles into tea. Environmental Science and Technology, 53(21), 12300-12310.
Hummel, D.O., 2002. Infrared Spectrometry. In: Atlas of Plastics Additives. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642- 56211-2_3.
Hussain, R., Khattak, S. A., Sattar, S., Ali, L., Ullah, Z., Muhammad, N., 2020 . Evaluation of the contaminated soil and its impacts on Tobacco (Nicotiana tabacum L.) crops in Swabi, Pakistan. Journal of Himalayan Earth Science, 53(1), 34-48.
Hwang, J., Choi, D., Han, S., Jung, S. Y., Choi, J., Hong, J., 2020. Potential toxicity of polystyrene microplastic particles. Scientific Reports, 10(1), 7391.
Iñiguez, M. E., Conesa, J. A., Fullana, A., 2017. Microplastics in Spanish table salt. Scientific Reports, 7(1), 8620.
Jung, M. R., Horgen, F. D., Orski, S. V., Rodriguez, V., Beers, K. L., Balazs, G. H., Lynch, J. M., 2018. Validation of ATR FT- IR to identify polymers of plastic marine debris, including those ingested by marine organisms. Marine Pollution Bulletin, 127, 704-716.
Koelmans, B., Pahl, S., Backhaus, T., Bessa, F., Calster, G.V., Contzen, N., Cronin, R., Galloway, T.S., Hart, A., Henderson, L., Kalčíková, G., Kelly, F.J., Kołodziejczyk, B., Marku, E., Poortinga, W., Rillig, M.C., Sebille, E.V., Steg, L., Steinhorst, J., Steidl, J., Syberg, K., Thompson, R.C., Wagner, M., Wezel, A.P., Wyles, K.J., Wright, S. L., 2019 . A Scientific Perspective on Microplastics in Nature and Society. SAPEA, Berlin, publisher 173 pp. Doi 10.26356/microplastics.
Khattak, M. S., Ali, S., 2015. Assessment of temperature and rainfall trends in Punjab province of Pakistan for the period 1961- 2014. Journal of Himalayan Earth Sciences, 48(2), 42-61.
Kutralam-Muniasamy, G., Pérez-Guevara, F., Elizalde-Martínez, I., Shruti, V. C., 2020. Branded milks–Are they immune from microplastics contamination. Science of the Total Environment, 714, 136823.
Leal Filho, W., Saari, U., Fedoruk, M., Iital, A., Moora, H., Klöga, M., Voronova, V., 2019. An overview of the problems posed by plastic products and the role of extended producer responsibility in Europe. Journal of Cleaner Production, 214, 550-558.
Li, Y., Peng, L., Fu, J., Dai, X., Wang, G., 2022.
A microscopic survey on microplastics in beverages: the case of beer, mineral water and tea. Analyst, 147(6), 1099-1105.
Liu, Y., Li, R., Yu, J., Ni, F., Sheng, Y., Scircle, A., Zhou, Y., 2021. Separation and identification of microplastics in marine organisms by TGA-FTIR-GC/MS: A case study of mussels from coastal China. Environmental Pollution, 272, 115946.
Łuczaj, W., Skrzydlewska, E., 2005. Anti- oxidative properties of black tea. Preventive Medicine, 40(6), 910-918.
MacLeod, M., Arp, H. P. H., Tekman, M. B., Jahnke, A., 2021. The global threat from plastic pollution. Science, 373(6550), 61-65.
Mason, S. A., Welch, V. G., Neratko, J., 2018. Synthetic polymer contamination in bottled water. Frontiers in Chemistry, 6, 389699.
Mehta, B. M., 2015. Chemical Composition of Milk and Milk Products. In: Cheung, P., Mehta, B. (eds) Handbook of Food Chemistry. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642- 36605-5_31
Mei, T., Wang, J., Xiao, X., Lv, J., Li, Q., Dai, H., Pi, F., 2022. Identification and evaluation of microplastics from tea filter bags based on Raman imaging. Foods, 11(18), 2871.
Mphaga, T., Mhlongo, T. N., Zikalala, S., Topkin, J. 2023. Identification and quantification of microplastics in waste water treatment plants by spectroscopic and microscopic techniques in Johannesburg East, South Africa. Water Practice and Technology, 18(12), 3124-3140.
Noda, I., Dowrey, A. E., Haynes, J. L., Marcott, C., 2007. Group Frequency Assignments for Major Infrared Bands Observed in Common Synthetic Polymers. In: Mark, J.E. (eds) Physical Properties of Polymers Handbook. Springer, New York, NY. https://doi.org/10.1007/978-0-387- 69002-5_22.
Obi, J., Yoshinaga, K., Tago, A., Nagai, T., Yoshida, A., Beppu, F., Gotoh, N., 2018. Simple quantification of lactones in milk fat by solvent extraction using gas chromatography– mass spectrometry. Journal of Oleo Science, 67(8), 941-948.
Oßmann, B. E., Sarau, G., Holtmannspötter, H., Pischetsrieder, M., Christiansen, S. H., Dicke, W., 2018. Small-sized microplastics and pigmented particles in bottled mineral water. Water Research, 141, 307-316. https://doi.org/10.1016/j.watres.2018.05. 027.
Qadeer, A., Anis, M., Warner, G. R., Potts, C., Giovanoulis, G., Nasr, S., Shuhang, W., 2024 . Global environmental and t o x i c o l o g i c a l d a t a o f e m e rg i n g plasticizers: current knowledge, regrettable substitution dilemma, green solution and future perspectives. Green Chemistry. https://doi.org/10.1039/D3GC03428C
Rameeza, F., Eun, J. B., 2022. Production, Consumption, and Trading of Tea in Pakistan. The Korean Tea Society, 28(2), 45-51. https://doi.org/10.29225/jkts.2022.28.2. 45
Schlawinsky, M., Santana, M. F., Motti, C. A., Martins, A. B., Thomas-Hall, P., Miller, M. E., Kroon, F. J., 2022. Improved microplastic processing from complex biological samples using a customized vacuum filtration apparatus. Limnology and Oceanography: Methods, 20(9), 553- 567.
Smith, M., Love, D. C., Rochman, C. M., Neff, R. A., 2018. Microplastics in seafood and the implications for human health. Current Environmental Health Reports, 5, 375-386.
Teboul, E., Orihel, D. M., Provencher, J. F., Drever, M. C., Wilson, L., Harrison, A. L., 2021 . Chemical identification of microplastics ingested by Red Phalaropes (Phalaropus fulicarius) using Fourier Transform Infrared spectroscopy. Marine Pollution Bulletin, 171, 112640.
Tejada-Casado, C., del Olmo-Iruela, M., García-Campaña, A. M., Lara, F. J., 2018. Green and simple analytical method to determine benzimidazoles in milk samples by using salting-out assisted liquid-liquid extraction and capillary liquid chromatography. Journal of Chromatography B, 1091 , 46 - 52 . https://doi.org/10.1016/j.jchromb.2018.0 5.024.
Tiwari, M., Rathod, T. D., Ajmal, P. Y., Bhangare, R. C., Sahu, S. K., 2019. Distribution and characterization of microplastics in beach sand from three different Indian coastal environments. Marine Pollution Bulletin, 140, 262-273.
Vikhareva, I. N., Aminova, G. K., Mazitova, A. K., 2021. Ecotoxicity of the adipate plasticizers: Influence of the structure of the alcohol substituent. Molecules, 26(16), 4833.
Xiang, J.; Liu, F.; Wang, B.; Chen, L.; Liu, W.; Tan, S., 2021. A Literature Review on Maillard Reaction Based on Milk Proteins and Carbohydrates in Food and Pharmaceutical Products: Advantages, Disadvantages, and Avoidance Strategies. Foods, 10(9), 1998. https://doi.org/10.3390/foods10091998
Zarus, G. M., Muianga, C., Brenner, S., Stallings, K., Casillas, G., Pohl, H. R., Gehle, K., 2023. Worker studies suggest unique liver carcinogenicity potential of polyvinyl chloride microplastics. American journal of industrial medicine, 66(12), 1033-1047.
